Next-Generation Protein Linkers: Enhancing Stability and Targeting in Bioengineered Therapeutics
Unmet Need
In protein bioengineering, stable and effective fusion proteins are crucial for therapeutic and diagnostic applications. However, current protein linkers often fail to maintain the stability, bioactivity, and targeted functionality needed, leading to limitations in drug efficacy and delivery, especially in challenging biological environments.
Technology
Our innovation is a library of protein linkers designed to improve the stability, folding, and bioactivity of fusion proteins. The library includes three classes of linkers—flexible, rigid, and cleavable—each addressing specific structural and functional requirements. Using Förster resonance energy transfer (FRET), we have characterized 39 linker variants with varying rigidity, allowing for tailored protein assemblies that enhance targeting and metabolic performance. This approach meets the demand for more effective bioengineered protein therapeutics and diagnostics by enabling optimized protein conformations and interaction.
Other Applications
Beyond therapeutic proteins, this linker technology can be adapted for molecular diagnostics and gene therapy, enhancing fusion stability in diagnostic probes and gene delivery vectors.
Advantages
- Enhances protein folding and stability
- Improves bioactivity and in vivo targeting
- Supports diverse applications (therapeutics, diagnostics, gene therapy)
- Provides flexibility with three classes of linkers