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Plasmids for the expression of B-arrestins and GRKs

Plasmids for the expression of B-arrestins and GRKs

Technology Duke inventors have generated 12 novel plasmids that can be used for the expression of β-arrestins and GRKs: pCDNA3-βarrestin1-Flag pCDNA3-βarrestin2-Flag pGEX-5G/LIC-βarrestin1 pGEX-5G/LIC-βarrestin2 pGEX-4T1-βarrestin1 C-terminus pGEX-4T1-βarrestin2 C-terminus pRK5-bGRK2 pRK5-bGRK3 pRK5-bGRK4 pRK5-hGRK5 pRK5-hGRK6 pRK5-hGRK26caax

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Hamster a1B-adrenergic receptor HeLa cell line

Hamster a1B-adrenergic receptor HeLa cell line

Technology Duke inventors have generated a human epithelial carcinoma (HeLa) cell line expressing the hamster α1B-adrenergic receptor.

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Conditional microRNA-9 inhibitor and reporter mouse

Conditional microRNA-9 inhibitor and reporter mouse

Technology Duke inventors have created a novel mouse model termed the conditional microRNA-9 inhibitor and reporter mouse that shows the level of microRNA-9 in all tissues and demonstrates a microRNA-9 deficiency phenoptype. When bred with…

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A mouse strain lacking functional GRK4 gene

A mouse strain lacking functional GRK4 gene

GRK4 is prominently expressed in testes, with expression also in select brain regions (cerebellum) and other organs and cell types. GRK4 has been implicated in regulation of cerebellar mGluRl and GABA, receptors, and may be…

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Rat a1A-adrenergic receptor HeLa cell line

Rat a1A-adrenergic receptor HeLa cell line

Technology Duke inventors have generated a human epithelial carcinoma (HeLa) cell line expressing the hamster α1B-adrenergic receptor.

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ZIP fish: zebrafish insulin promoter

ZIP fish: zebrafish insulin promoter

Duke scientists have created stable transgenic zebrafish with strict pancreatic, beta cell-specific expression of GFP in embryos, larvae and adult. This model can serve as a high throughput screen for diabetic drugs.

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Transgenic zebrafish with hand2 tagged with GFP

Transgenic zebrafish with hand2 tagged with GFP

Technology Duke inventors have generated transgenic zebrafish with the native hand2 developmental transcription factor tagged with green fluorescent protein (GFP). This allows visualization of the activation of hand2 regulatory sequences and can be used for…

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Activated caspase-3 reporter mouse

Activated caspase-3 reporter mouse

Technology The activated caspase-3 reporter mouse is an in vivo screening assay for apoptosis (regulated, energy-dependent cell death) in all tissues. Fluorescence miscroscopy of the fluorophore mCerulean reveals levels of caspase-3 activation in cells, and…

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Dopamine transporter knockout mice as a screening tool for anti-parkinsonian and anti-dyskinetic agents

Dopamine transporter knockout mice as a screening tool for anti-parkinsonian and anti-dyskinetic agents

Unmet Need The dopamine precursor L-DOPA is the primary treatment for Parkinson's Disease (PD). However, in addition to alleviating motor deficits associated with PD, 50-90% of patients experience side effects such as dyskinesia after 5-10…

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Transgenic mouse strains to investigate biased ghrelin receptor (GHSR1a) drug candidates

Transgenic mouse strains to investigate biased ghrelin receptor (GHSR1a) drug candidates

Technology Duke inventors have created five novel transgenic mice with point, knockout, or humanizing mutations to the ghrelin receptor (GHSR1a): G protein-biased GHSR1a mouse (GHSR-P147A): CRISPR-Cas9 RNA-induced homology-directed repair (HDR) was used to create a…

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An induced pluripotent stem cell model of Duchenne muscular dystrophy

An induced pluripotent stem cell model of Duchenne muscular dystrophy

Unmet Need Duchenne muscular dystrophy (DMD) is a rare genetic disease that causes the skeletal and heart muscles to weaken. Due to a mutation in dystrophin, an important protein involved in muscle structure, individuals with…

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Cell line expressing chimeric Tie2 receptors

Cell line expressing chimeric Tie2 receptors

Technology Duke inventors have generated a cell line that expresses chimeric Tie2 receptors. Specifically, NIH 3T3 cells were infected with retroviruses containing a plasmid that encoded the extracellular and transmembrain domains of the c-fms receptor…

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